Service Detail

 

Product No.

Service Name and Description

Turnaround Time / Remarks

SS3100

Human Cell Line Authentication

Genetic profiling of species specified short tandem repeat (STR) markers

Deliverable: Report and raw loci calls

3 to 5 working days

 

Sample Requirement

 
Product Sample Type Sample Requirements

Purified Genomic DNA

>10ng/ul in 10ul

Cell Pellet*

  1. Grow cell in T25 Flask (or equivalent) until 70% confluent.
     
  2. Harvest cells and make aliquots into 2 to 3 tubes
     
  3. Wash with 1x PBS.
     
  4. Store and ship cell pellets in dry ice.

*gDNA extraction charge applies

FTA Card*
  1. Prepare samples one at a time at an optimal target cell density of 1 x 106 cells/ml.
    a. For attached cells: After trypsinization, wash cells with 1X PBS. Re-suspend the cells in a small volume of PBS, count the cells and dilute the sample to 1 x 106 cells/ml.
    b. For suspension cells: Harvest and count cells.
    < ! If cell density is less than 1 x 106 cells/ml, must re-centrifuge and re-suspend in PBS to get a final concentration of 1 x 106 cells/ml. If cell density is greater than 1.7 x 106 cells/ml, then dilute the sample in PBS to 1 x 106 cells/ml>
     
  2. Clean the work surface in a cell culture hood, open sample storage card with gloved hands.
     
  3. Label the sample storage card. Spot 40 μl of cell suspension from step 1 in the center of each circle of the sample storage card, total 2 - 4 circles per sample.
     
  4. Air dry sample storage card at room temperature.
     
  5. Close sample storage card and put it inside the collection bag (optional with desiccant pack). Ship at room temperature.

 

*gDNA extraction charge applies

   

 

Order form 

Please send the completed the Order form to our respective email accounts to activate a free local collection. For international orders, please send us an email with your shipment tracking number to ensure speedy processing of your orders.

 

Location

Singapore

Malaysia

International

Email to:

sequencing@axilscientific.com

sequencing@apicalscientific.com

sequencing@apicalscientific.com

 Tel:

 +65 67757318

 +60-3-8943 3252

 +60-3-8943 3252