| Product No. |
Name of Service |
Service Includes |
Suited for |
| Protein Identification Services |
| SS001-1 |
MALDI-TOF/TOF mass spectrometry (Sequencing by MS/MS) |
Trypsin digestion of protein, running of samples with automatic database analysis. |
Optimal for pure protein from 2D gel spots |
| SS001-2 |
Electrospray (LC/MS/MS) Mass Spectrometry |
Trypsin digestion of protein, running of samples with automatic database analysis. |
Optimal for a single gel band containing 2 or more proteins, or low abundant samples. |
| SS002 |
De Novo Peptide Sequencing by Mass Spectrometry. |
MALDI TOF/TOF is used to determine the primary sequence structure of proteins. Manual interpretation of the MS/MS spectra and derivation of internal sequence will be done using the latest software. |
Optimal for pure protein from 2D gel spots, and does not have the related genome from database. |
SS002-1
|
De Novo Peptide Sequencing by Mass Spectrometry. |
MALDI TOF/TOF is used to determine the primary sequence structure of proteins. Manual interpretation of the MS/MS spectra and derive of internal sequence will be done by the expert operator using the latest software. Price per sample inclusive of 1 peptide sequence for de novo search.
|
Optimal for 2D gel spot with coomassive stain only and available only for pure protein stained with Coomasive blue, that does not have the related genome from database.
|
SS002-2
|
De Novo Peptide Sequencing by Mass Spectrometry. |
LC/MS/MS is used to determine the primary sequence structure of proteins. Manual interpretation of the MS/MS spectra derived from internal sequence will be done by the expert operator using the latest software. Price per sample inclusive of 1 peptide sequence for de novo search
|
Only available for pure protein stained with Silver or other MALDI compatible stains eg.Ruby or Cy dyes, that does not have the related genome from database. |
SS002-3
|
De Novo Peptide Sequencing, for each additional peptide. |
Additional peptide sequence needed for De Novo Peptide Sequencing Service
|
For de novo samples that require probe/primer design, a minimum of 3 peptide sequences for de novo search is recommended. |
| SS010-1 |
N-Terminal Protein Sequencing, first 3 amino acid residues. |
Edman degradation. The first 3 amino acid residues is also the basic fee if the N-terminal is blocked (which is the no result fee). |
Pure proteins that require definitive sequence of the amino acid from the N-terminus. |
| SS010-2 |
N-Terminal Protein Sequencing, each additional amino acid residues |
Edman degradation. Minimum 12 residues are recommended for protein identification. 15 residues are highly recommended (up to 30 residues) |
| Proteome Mapping – MuDPIT Analysis |
| SS003-1 |
Proteome Mapping – MuDPIT analysis: 1D LC-MALDI. Price per Sample. |
Simple protein sample is run through an extended LC gradient, and the eluent spotted for MALDI-TOF/TOF analysis. |
Optimal for simple proteins. It allows identification up to 100 spots per experiment. |
| SS003-2 |
Proteome Mapping – MuDPIT analysis: 2D LC-MALDI. Price per Sample. |
Complex protein sample is run through a sophisticated 2D LC gradient, and the eluent spotted for MALDI-TOF/TOF analysis. |
Optimal for complex proteins. It allows identification up to1000 spots per experiment. |
| Protein Sample Preparation Services |
| SS004-1 |
2-Dimensional gel electrophoresis. Large gel at price per gel. |
Including 1st, 2nd dimension gels and staining (silver/ Coomassie) |
Crude proteins usually acquire 4 gels per experiment. |
| SS004-2 |
2-Dimensional gel electrophoresis. Small gel at price per gel. |
Including 1st, 2nd dimension gels and staining (silver/ Coomassie) |
| SS004-3 |
2-Dimensional gel electrophoresis service: High sensitivity Sypro staining. Price per gel. |
High sensitivity of Sypro staining. |
For weak samples that require staining others than Coomassive and Silver staining. |
| SS004-4 |
2-Dimensional gel electrophoresis service: Image analysis and spot recognition (Client gels) by Progenesis Same Spots. Set of four replicate gels. Price per gel. |
Image analysis and spot recognition |
Recommended for crude proteins that require both 2D services and image analysis. |
| SS004-5 |
2-Dimensional gel electrophoresis service: Pattern comparison. Two sets of four replicate gels. Price per set. |
Gel replicates merged to prepare master gels, which are compared in pairwise sets. |
Recommended for crude proteins that require both 2D services and image comparison between other protein(s) that submitted together. |
| SS004-6 |
2-Dimensional gel electrophoresis service: Blotting of protein from 2D PAGE gels onto sequencing grade PVDF membrane. Price per blot. |
We now can accept protein samples from 2D samples that run by us for N-terminal protein sequencing, without the need of blotting them onto PVDF membrane. |
This is only recommended for the customer that needs their protein to be run on 2D, and return the protein on PVDF membrane. |
| Protein Differential Expression – iTRAQ Analysis |
SS005-1 |
iTRAQ Enhanced Analysis Service |
Sample labelling and analysis by
(i) 1D LC-MALDI mass spectrometry to understand the sample complexity
(ii) 2D LC-MALDI mass spectrometry, with automatic database analysis (replicate, up to 4 samples)
Price excludes dry-ice shipment.
|
Minimum 2 different protein extracts for one iTRAQ analysis (1 protein extract is the control/ reference, another 1 protein extract is the targeted sample for differential expression comparison) |
| SS005-1-Proviso |
Minimum setup for iTRAQ Enhanced Analysis Service |
Setup fee will apply if sample(s) failed to proceed to 2D LC-MALDI mass spectrometry. Price excludes dry-ice shipment |
|
| SS005-2 |
iTRAQ Analysis Service (Re-run) |
Sample labelling and analysis by 2D LC-MALDI mass spectrometry, with automatic database analysis.
Price for repeat experiment from SS005-1 (replicate, up to 4 samples), excludes dry-ice shipment.
|
Proteins that are extracted in the different period may have different expression profile. This service is recommended for re-run of same protein extracts that were already analysed in SS005-1. |
| Protein Mass and Component Analysis |
| SS006-1 |
Multiple Reaction Monitoring (MRM) analysis: Method development. Price per day. |
Estimate 2 to 5 days are required for each method. |
Biomarker analysis and reaction monitoring in pharmacokinetic studies for toxic metabolites. |
| SS006-2 |
Multiple Reaction Monitoring (MRM) analysis: Data collection and analysis. Price per sample |
Data collection and analysis performed as agreed in the project/ method from SS006-1. |
|
| SS007 |
Phosphorylation Detection. Price per sample |
Precursor ion and neutral loss scans that allow detection of the loss of the phosphate group. |
Pure protein and peptide samples required identification of phosphorylation sites. These sites can be mapped to detect the important post-translational modification of proteins. |
| SS008-1 |
Protein Mass analysis: peptide (<15 kDalton). Price per peptide. |
Samples are processed intact (no enzyme digestion). Intact mass by MALDI-TOF mass spectrometry. |
Pure peptide (<15 kDalton). Most of the peptide has mass < 15 kDalton. |
| SS008-2 |
Protein Mass analysis: protein (<15 kDalton). Price per protein. |
Samples are processed intact (no enzyme digestion). Intact mass by MALDI-TOF mass spectrometry. |
Pure protein (<15 kDalton). |
| SS008-3 |
Protein Mass analysis: peptide (>15 kDalton). Price per peptide. |
Samples are processed intact (no enzyme digestion). Intact mass by electrospray mass spectrometry. |
Pure peptide (>15 kDalton) |
| SS008-4 |
Protein Mass analysis: protein (>15 kDalton). Price per protein. |
Samples are processed intact (no enzyme digestion). Intact mass by electrospray mass spectrometry. |
Pure protein (>15 kDalton). Most of the protein has mass > 15 kDalton. |
| SS009-1 |
Amino Acid Analysis: Peptide. Price per sample. |
Chemical hydrolysis up to 18 amino acids per analysis. Not included Cystein and Tryptophan detection. |
Ideal for quality control and the amino acid composition of peptides. |
| SS009-2 |
Amino Acid Analysis: Protein. Price per sample. |
Chemical hydrolysis up to 18 amino acids per analysis. Not included Cystein and Tryptophan detection. |
Ideal for quality control, the amino acid composition of protein and peptide samples (include recombinant proteins from cell culture and fermentation material, nutraceuticals and plant materials). |
| SS009-3 |
Amino Acid Analysis: Determination of Cystein per analysis. Price per sample. |
Special hydrolysis method to detect only Cystein. |
Peptide/ protein sample(s) that specified for Cystein detection. |
| SS009-4 |
Amino Acid Analysis: Determination of Tryptophan per analysis. Price per sample. |
Special hydrolysis method to detect only Tryptophan. |
Peptide/ protein sample(s) that specified for Tryptophan detection. |
| Others |
| - |
Project Consultation |
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Please enquire |
