Fluorogenic Probes

Quantitative Real-Time PCR is now used routinely as an important analytical tool in the life sciences. Quantitative Real-Time PCR allows for Gene Expression Profiling and Real-time Quantification, to provide data for the following applications.

1. Gene expression profiling

   • Mutation detection
   • Allelic discrimination
   • Single Nucleotide Polymorphisms (SNPs)
   • Pathogen detection

2. Quantification

   • Melting curves profiles
   • Single or multiplex assays
   • Universal cycling conditions
   • High-throughput screening assays

1st BASE provides high quality Dual-Labeled Hydrolysis Probes used for routine qPCR, these are available as DNA or DNA mixed with LNA bases.

Features of Dual-Labeled Hydrolysis DNA Probes

• Available in 3 scales (100 nmole, 200 nmole and 1.0 umole)
  
• HPLC purified for up to 95% purity
• 100% QC-checked by HPLC and Mass Spectrometry
• Delivered within 1 week (Extended delivery for probes of higher synthesis scale)
• Standard pricing for up to 35 mers in length. (Please enquire if probe sequences exceed 35-mers)