Illumina sequencing technology

The Illumina sequencing platform relies on parallel sequencing of millions of fragments using their proprietary reversible terminator-based sequencing chemistry. This offers a highly robust, accurate, and scalable system.

1. An adaptor-ligated DNA library is prepared, and attached to a planar, optically transparent surface.
   
2. The attached DNA fragments are extended and bridge-amplified, creating a flow-cell with hundreds of millions of clusters of DNA. Each cluster contains thousands of copies of the DNA template.
   
3. These templates are sequenced using a robust four-color DNA sequencing-by-synthesis technology that employs reversible terminators with removable fluorescent dyes.

Specifications
Read Lengths: Up to 100bp
Reads per run / Throughput: 180-200M per flowcell
System Accuracy: Greater Raw read accuracy of 99% and above
Quality Score: 70-90% of base calls at >QV30
Information taken from http://www.illumina.com/technology/sequencing_technology.ilmn, accurate as of June 2010.

Applications

• Sequencing
  
• Genotyping and CNV analysis
  
• Gene Regulation and Epigenetic Analysis