RNA Interference and siRNA
RNA Interference is a mechanism for RNA-guided regulation of gene expression in which double-stranded ribonucleic acid inhibits the expression of genes with complementary nucleotide sequences. It is a naturally occurring cellular mechanism and can be used by researchers to specifically target and silence any gene of interest.
After Tuschl et al's keynote paper in Cell, 2000 on how 21-23mer double-stranded RNA (dsRNA) directs the sequence-specific degradation of mRNA leading to specific gene knockdown, a wave of research emerged using short interfering RNA (siRNA) and RNAi as a tool to study Functional Genomics, Developmental Biology, Gene Expression and in vivo and in vitro Transfection Efficiency studies.
There are various approaches to study RNAi, synthetic siRNAs and shRNA constructs are 2 of the numerous options available. 1st BASE provides the custom synthesis of sequence-specific siRNA for direct transfection, as well as the routine synthesis of shRNA oligos, ~60 bases long purified DNA oligos to be cloned to form shRNA constructs. See DNA Synthesis for information on DNA oligos.
Features of custom siRNA synthesis
- Our siRNA duplexes are HPLC purified and annealed before delivery. siRNA orders are supplied lyophilised, and delivered with DEPC - treated water for reconstitution.
- Chemically modified siRNA duplexes, such as fluorescent 6-FAM labels are also available. These RNAi duplexes can come as 19 to 28bp duplexes with UU or NN overhangs.
- 1st BASE will provide complimentary siRNA duplex design assistance for confirmed orders. Simply provide the Gene Accession number of your Gene of Interest. We will provide our siRNA proposals upon confirmation of your purchase. It is highly recommended to take up 2 - 3 designs to ensure a higher success rate of gene knockdown.
List of 1st BASE siRNA Products
| Product No | Description |
|---|---|
| siRNA Duplex - 5 OD | siRNA Oligo (duplex) 5 OD yield, HPLC Purified (97% purity) |
| siRNA Duplex - 10 OD | siRNA Oligo (duplex) 10 OD yield, HPLC Purified (97% purity) |
| siRNA Duplex - 25 OD | siRNA Oligo (duplex) 25 OD yield, HPLC Purified (97% purity) |
| siRNA Duplex 6FAM- 5 OD | siRNA Oligo (duplex), 5' 6FAM label on sense
strand 5 OD yield, HPLC Purified (97% purity) |
| siRNA Negative Control - 1 OD | siRNA Negative Control (21mer duplex), HPLC purified, 1 OD yield |
| siRNA Negative Control - 5 OD | siRNA Negative Control (21mer duplex), HPLC purified, 5 OD yield |
| siRNA Positive Control - 1 OD | siRNA Positive Control (21mer duplex), HPLC purified, 1 OD yield |
| siRNA Positive Control - 5 OD | siRNA Positive Control (21mer duplex), HPLC purified, 5 OD yield |
**For larger scale syntheses or alternative modifications, please send your enquiries to oligo-sg@base-asia.com
Published and Validated siRNA Controls: In order to conduct a quantifiable knockdown experiment, it is crucial to set up control reactions (positive, negative and transfection controls). 1st BASE has a comprehensive range of ready-made validated siRNA controls to help researchers set up their controls with ease!
| Gene Name | Gene Name |
|---|---|
| CHO1 | keratin 18 |
| corin, mouse | hKIS (Kinase interacting stathmin) |
| Cyclin B1 | Lamin A |
| Cyclin B2 | Lamin A/C |
| Cytoplasmic Dynein 1 Heavy Chain | Lamin B1 |
| DCC interacting protein ( DIP13-alpha) | Lamin B2 |
| Dishevelled (hDv-1 and hDv-3) | LAP2 |
| Eg5 | Mad1 |
| Emerin | MSF (Mammalian septin) |
| Eve | MeCP2 (Methyl-CpG-binding protein 2) |
| Fortilin | MBD2 (Methyl-DpG binding domain protein 2) |
| GalTII (galactosyltransferase II) | hMps1 (MPS1 kinase) |
| GAS41 (1) | MCL1 (Myeloid cell leukemia protein 1) |
| hDv-2 | Nct (Nicastrin) |
| Hec-1 | NuMA |
| hFbw7 (cyclin E ubiquitination) | Nup153 |
| HPV E6 | P160 ROCK |
| HPV E7 | hP1k1 (Polo-like Kinase1) |
| hRad9 | PKR (Protein Kinase Receptor) |
| HtrA2 | Rad17 |
| hTF (Tissue Factor) | Vimentin |
1st BASE is continuously adding on to this list, please contact us for the most updated list of siRNA controls.