Dual-labeled Fluorogenic Probes for Real-Time PCR
Quantitative Real-Time PCR is now used routinely as an important analytical tool in the life sciences. Quantitative Real-Time PCR allows for Gene Expression Profiling and Real-time Quantification, to provide data for the following applications.
-
Gene expression profiling
- Mutation detection
- Allelic discrimination
- Single Nucleotide Polymorphisms (SNPs)
- Pathogen detection
-
Quantification
- Melting curves profiles
- Single or multiplex assays
- Universal cycling conditions
- High-throughput screening assays
1st BASE provides high quality Dual-Labeled Fluorogenic Probes used for routine qPCR, available as DNA and LNA Dual-Labeled Fluorogenic Probes.
Features of Dual-Labeled Fluorogenic DNA Probes 1st BASE provides high quality Dual-Labeled Fluorogenic Probes used for routine qPCR. The standard length of DNA Dual-Labeled probes is between 18 to 35 bases and the probes are HPLC purified and quality checked by Mass Spectrometry. 1st BASE Dual-Labeled DNA Probes are synthesize at 50nmole, 200nmole and 1 umole scale with the respective yield of 2 OD, 5 OD and 10 OD. The turnaround time of 7 working days applies for standard probes.
Standard Dual-Labeled Fluorogenic DNA Probes.
| Description | Synthesis Scale | Yield Guarantee |
|---|---|---|
| Dual-Labeled DNA Probe 1 5' 6-FAM, HEX, TET & 3' TAMRA or Dabcyl | 50 nmole scale, HPLC Purified | Guaranteed 1 OD |
| Dual-Labeled DNA Probe 1 5' 6-FAM, HEX, TET & 3' TAMRA or Dabcyl | 200 nmole scale, HPLC Purified | Guaranteed 1 OD |
| Dual-Labeled DNA Probe 1m 5' 6-FAM, HEX, TET & 3' TAMRA or Dabcyl | 1.0 umole scale, HPLC Purified | Guaranteed 3 OD |
| Dual-Labeled DNA Probe 2 5' 6-FAM, HEX, TET & 3' BHQ-1 | 50 nmole scale, HPLC Purified | Guaranteed 1 OD |
| Dual-Labeled DNA Probe 2 5' 6-FAM, HEX, TET & 3' BHQ-1 | 200 nmole scale. HPLC Purified | Guaranteed 2 OD |
| Dual-Labeled DNA Probe 2 5' 6-FAM, HEX, TET & 3' BHQ-1 | 1.0 umole scale, HPLC Purified | Guaranteed 8 OD |
| Dual-Labeled DNA Probe 3 5' Cy5, Cy3, TAMRA & 3' BHQ-2 | 50 nmole scale, HPLC Purified | Guaranteed 1 OD |
| Dual-Labeled DNA Probe 3 5' Cy5, Cy3, TAMRA & 3' BHQ-2 | 200 nmole scale, HPLC Purified | Guaranteed 1 OD |
| Dual-Labeled DNA Probe 3 5' Cy5, Cy3, TAMRA & 3' BHQ-2 | 50 nmole scale, HPLC Purified | Guaranteed 1 OD |
| Dual-Labeled DNA Probe 4 5' Texas Red or ROX & 3' Dabcyl or BHQ-2 | 200 nmole scale, HPLC Purified | Guaranteed 4 OD |
| Dual-Labeled DNA Probe 4 5' Texas Red or ROX & 3' Dabcyl or BHQ-2 | 1.0 umole scale, HPLC Purified | Guaranteed 12 OD |
Dual-Labeled Fluorogenic Probes with LNA bases Introducing LNA bases into a qPCR probe in our Dual-Labeled Fluorogenic LNA Probes brings several advantages such as:
- Increased thermal stability and hybridization specificity
- More accurate gene quantitation and allelic discrimination
- Easier and more flexible probe designs for problematic target sequences
These technological advantages are due to the following features:
- Higher Tm by LNA contribution increasesthermal stability and hybridization specificity
- More accurate gene quantitation and allelic discrimination improves SNP detection assays
- Shorter LNA probes enable easier and more flexible probe designs for problematic target sequences
- Compatible with real-time PCR platforms and end-point analytical detection instruments
- Incorporation of LNA monomers by standard phosphoramidite chemistry allows the use of the same synthesis/modification protocols as DNA and RNA bases
1st BASE will provide complimentary hydrolysis probe design
assistance for confirmed probe and primer sets orders.
Simply write
to us at
oligo-sg@base-asia.com
and include the Gene Accession number of your Gene of Interest.
We
will provide our proposals within 3 working days, upon your
confirmation of purchase.
For larger scale syntheses or alternative dyes / modifications, please contact us at oligo-sg@base-asia.com