Single Pass DNA Sequencing (SS1001, SS1002)
A full service DNA sequencing reaction encompasses the cycle sequencing reactions, dye terminator removal and subsequent analysis on our Genetic Analyzers. We also accept samples in 96-well plates. This is recommended for bulk orders, with a minimum of 80 reactions per plate.
Customers need to send us their purified DNA (plasmid or PCR product) and specific primers individually. We strongly recommend 1.5mL of microtube to be used for Service Code SS1001; and PCR strip-capped 96-well plate for Service code: SS1002
Sample Preparation Requirements for Local Customers (Singapore and Malaysia) are as follows:
| Type of Primer |
Concentration & Volume/ Reaction |
| Primer |
10µM or 10pmol/µL, in 5µL per rxn in dH2O |
| 30µM or 30pmol/µL, in 5µL per rxn in dH2O (Applicable for Direct Genomic Sequencing only) |
Sample Preparation Requirements for International Customers are as follows:
We recommend that international customers send a larger volume of their samples to overcome loss of samples due to evaporation during the extended transportation to our facilities.
| Type of DNA |
Concentration & Volume/ Reaction |
| Purified PCR product [90-250bp] |
[90-250bp] 10 ng/µL, min 10 µL per rxn in dH2O |
| Purified PCR product [251-500bp] |
[251-500bp] 20 ng/µL, min 10 µL per rxn in dH2O |
| Purified PCR product [>500bp] |
[>500bp] 40 ng/µL, min 10 µL per rxn in dH2O |
| Purified Plasmid |
100 ng/µL, min 10 µL per rxn in dH2O |
| Purified M13/phagemid ssDNA |
50 ng/µL, min 10 µL per rxn in dH2O |
| Purified Genomic DNA |
400 ng/µL, min 20 µL per rxn in dH2O |
| Type of Primer |
Concentration & Volume/ Reaction |
| Primer |
10µM or 10pmol/µL, in 5µL per rxn in dH2O |
| 30µM or 30pmol/µL, in 5µL per rxn in dH2O (Applicable for Direct Genomic Sequencing only) |
The following list of Universal Primers is provided by our DNA Sequencing facility at no cost.
| Universal Primers |
Sequence (5' - 3') |
| M13F (-20) |
5' GTAAAACGACGGCCAGT 3' |
| M13F (-29) |
5' CACGACGTTGTAAAACGAC 3' |
| M13F-pUC (-40) |
5' GTTTTCCCAGTCACGAC 3' |
| M13R (-20) |
5' GCGGATAACAATTTCACACAGG 3' |
| M13R (-24) |
5' GGAAACAGCTATGACCATG 3' |
| M13R-pUC (-26) |
5' CAGGAAACAGCTATGAC 3' |
| T7 |
5' AATACGACTCACTATAG 3' |
| T7 Promoter |
5' TAATACGACTCACTATAGGG 3' |
| T7 Terminator |
5' GCTAGTTATTGCTCAGCGG 3' |
| T3 |
5' ATTAACCCTCACTAAAG 3' |
| SP6 |
5' ATTTAGGTGACACTATAG 3' |
| CMV Forward |
5' CGC AAA TGG GCG GTA GGC GTG 3' |
| BGH-rev |
5' CTAGAAGGCACAGTCGAGGC 3' |
| AOX1 Forward |
5' GACTGGTTCCAATTGACAAGC 3' |
| AOX1 Reverse |
5' GCAAATGGCATTCTGACATCC 3' |
| pBRrevBam |
5' GGTGATGTCGGCGATATAGG 3' |
|
