Single Pass DNA Sequencing(SS1001, SS1002)
A full service DNA sequencing reaction encompassing the cycle sequencing reactions, dye terminator removal and subsequent analysis on our capillary electrophoretic analyzer.
Also accepted: samples in 96-well plates. This is recommended for bulk orders, with a minimum of 80 reactions per plate.
Customers need to send us their purified DNA (plasmid or PCR product) and specific primers. Results may be expected in 2 - 3 working days upon order acknowledgement.
Sample Preparation Requirements for Local Customers (Singapore and Malaysia)
| Type of DNA | Concentration & Volume/ Reaction |
|---|---|
| Purified PCR product [90-250bp] | 10 ng/µL, min 10 µL per rxn in dH2O |
| Purified PCR product [251-500bp] | 20 ng/µL, min 10 µL per rxn in dH2O |
| Purified PCR product [>500bp] | 40 ng/µL, min 10 µL per rxn in dH2O |
| Purified Plasmid | 100 ng/µL, min 10 µL per rxn in dH2O |
| Purified M13/phagemid ssDNA | 50 ng/µL, min 10 µL per rxn in dH2O |
| Purified Genomic DNA | 400 ng/µL, min 20 µL per rxn in dH2O |
| Type of Primer | Concentration & Volume/ Reaction |
|---|---|
| Primer | 10µM or 10pmol/µL, in 5µL per rxn in dH2O |
| 30µM or 30pmol/µL, in 5µL per rxn in dH2O (Applicable for Direct Genomic Sequencing only) |
Sample Preparation Requirements for International CustomersWe recommend that international customers send a larger volume of their samples to overcome loss of samples due to evaporation during the extended transportation to our facilities.
| Type of DNA | Concentration & Volume/ Reaction |
|---|---|
| Purified PCR product [90-250bp] | 10 ng/µL, min 20 µL per rxn in dH2O |
| Purified PCR product [251-500bp] | 20 ng/µL, min 20 µL per rxn in dH2O |
| Purified PCR product [>500bp] | 40 ng/µL, min 20 µL per rxn in dH2O |
| Purified Plasmid | 100 ng/µL, min 20 µL per rxn in dH2O |
| Purified M13/phagemid ssDNA | 50 ng/µL, min 20 µL per rxn in dH2O |
| Purified Genomic DNA | 400 ng/µL, min 30 µL per rxn in dH2O |
| Type of Primer | Concentration & Volume/ Reaction |
|---|---|
| Primer | 10µM or 10pmol/µL, in 10µL per rxn in dH2O |
| 30µM or 30pmol/µL, in 10µL per rxn in dH2O (Applicable for Direct Genomic Sequencing only) |
The following list of Universal Primers is provided by our DNA Sequencing facility at no cost.
| Universal Primers | Sequence (5' - 3') |
|---|---|
| M13F (-20) | 5' GTAAAACGACGGCCAGT 3' |
| M13F (-29) | 5' CACGACGTTGTAAAACGAC 3' |
| M13F-pUC (-40) | 5' GTTTTCCCAGTCACGAC 3' |
| M13R (-20) | 5' GCGGATAACAATTTCACACAGG 3' |
| M13R (-24) | 5' GGAAACAGCTATGACCATG 3' |
| M13R-pUC (-26) | 5' CAGGAAACAGCTATGAC 3' |
| T7 | 5' AATACGACTCACTATAG 3' |
| T7 Promoter | 5' TAATACGACTCACTATAGGG 3' |
| T7 Terminator | 5' GCTAGTTATTGCTCAGCGG 3' |
| T3 | 5' ATTAACCCTCACTAAAG 3' |
| SP6 | 5' ATTTAGGTGACACTATAG 3' |
| CMV Forward | 5' CGC AAA TGG GCG GTA GGC GTG 3' |
| BGH-rev | 5' CTAGAAGGCACAGTCGAGGC 3' |
| AOX1 Forward | 5' GACTGGTTCCAATTGACAAGC 3' |
| AOX1 Reverse | 5' GCAAATGGCATTCTGACATCC 3' |
| pBRrevBam | 5' GGTGATGTCGGCGATATAGG 3' |