| Product No. |
Name of Service |
Service Requirements |
Turnaround Time |
| DNA Sequencing Services |
| SS1001 |
Single Pass DNA Sequencing |
Customer to provide purified PCR product(s) or purified plasmid(s) with sequencing primer(s) in separate tubes. |
1-2 working days |
| SS1002 |
Single Pass DNA Sequencing, 96-well format |
High Throughput (HT) DNA Sequencing. Leave well H12 empty for our control reaction, Each plate comprises of maximum 95 samples with normalized OD. |
2-3 working days |
| SS1003 |
Single Pass DNA Sequencing with Quantification |
Similar to SS1001, except that an additional charge will be levied for agarose gel electrophoresis quantification. |
1-2 working days |
| SS1004 |
Ready Mix DNA Sequencing |
Customer to provide purified PCR product(s) or purified plasmid(s) with sequencing primer(s) in the same tube. 1 tube = 1 reaction. |
1-2 working days |
| SS1005 |
Ready to Load Sequencing |
We accept reactions done using BigDye® v3.0 or v3.1. |
1-2 working days |
| SS1006 |
Difficult Template Sequencing |
Refers to DNA templates with 70% GC-rich/ 70% AT-rich/ GT-rich/ repetitive sequences. |
2-3 working days |
| SS1007 |
Bi-Directional DNA Sequencing |
Customer to provide purified PCR product(s) or purified plasmid(s) with sequencing primer(s) in separate tubes. 1 service comprises of 2 single pass sequencing reactions from the same DNA template. |
1-2 working days |
| SS1008 |
Primer Walking of Constructs Service - Single Pass. |
Charge per bp of data. Sequencing runs bi-directionally until a length of overlapping sequences is obtained. Customer to provide vector name and RE cut sites (where applicable) for data alignment. Service inclusive of primer design, primer synthesis, DNA sequencing and data alignment. Will be charged as per SS1001, if sequencing cannot proceed beyond the first round using Universal Primers. |
1.5 kb per week |
| SS1009 |
Primer Walking of Constructs Service - Bi-Directional. Charge per bp of data. |
Sequencing runs bi-directionally until the end of insert. Customer to provide vector name and RE cut sites (if applicable) for data alignment. Service inclusive of primer design, primer synthesis, sequencing and data alignment. Will be charged as per SS1001, if sequencing cannot proceed beyond the first round using Universal Primers. 1.5 kb per week
SS1010 Direct Genomic Sequencing Customer to provide purified genomic DNA directly (without additional PCR) and its specific sequencing primer. Sample purity and priming efficiency of its sequencing primer are crucial to the outcome of the sequencing reaction. |
2-3 working days |
| SS1010 |
Direct Genomic Sequencing |
Customer to provide purified genomic DNA directly (without additional PCR) and its specific sequencing primer. Sample purity and priming efficiency of its sequencing primer are crucial to the outcome of the sequencing reaction. |
2-3 working days |
| SS1011 |
BAC (Bacteria Artificial Chromosome) End Sequencing |
BAC holds large genomic DNA constructs, ranging from 150 to 350 kbp. Customer to provide purified BAC and its specific sequencing primer (where applicable). Sample purity is crucial to the outcome of the sequencing reaction. |
2-3 working days |
| Sample Preparation and Quantification Service |
| SS1012 |
DNA Sequencing Sample Preparation - PCR Clean-up |
PCR purification by spin column method. Spin column is only recommended for single band-product. |
1-2 working days |
| SS1013 |
DNA Sequencing Sample Preparation - PCR Clean-up 96-well format |
PCR purification by spin column in 96-well plate. Spin column is only recommended for single band-product. |
2-3 working days |
| SS1014 |
DNA Sequencing Sample Preparation - Gel Extraction by General Agarose |
PCR purification by gel extraction using general agarose gel. Customer to indicate the fragment size to be excised. |
1-2 working days |
| SS1015 |
DNA Sequencing Sample Preparation - Gel Extraction by General Agarose 96-well format |
High throughput PCR purification by gel extraction using general agarose gel. Customer to indicate the fragment size to be excised. |
2-3 working days |
| SS1016 |
DNA Sequencing Sample Preparation - Gel Extraction by PAGE |
PCR purification by gel extraction using PAGE gel. Customer to indicate the fragment size to be excised. |
1-2 working days |
| SS1017 |
DNA Sequencing Sample Preparation - Plasmid Extraction |
Customer to provide colony plate prepared from single colony, vector name, antibiotic resistant and concentration of antibiotic. Fresh colony plate (not more than 2 weeks) or fresh broth (not more than 1 month) is recommended. |
2-3 working days |
| SS1018 |
DNA Sequencing Sample Preparation - Plasmid Extraction 96-well format |
Customer to provide 96-deep well plate containing culture broth from single colony, vector name, antibiotic resistant and concentration of antibiotic. Fresh broth (not more than 1 month) is recommended. |
2-3 working days |
| SS1019 |
Ready to load Sequencing with Clean-up |
Customer to provide fresh BigDye® reaction. We will perform the clean-up and precipitation steps of the BigDye® reaction before loading on our Genetic Analyzer. |
1 working day |
| SS1020 |
DNA Sequencing Sample Quantification - By Agarose Gel |
This service allows customers to skip the hassle of gel electrophoresis to check sample purity and concentration before cycle sequencing. Combination of SS1020 and SS1021 is often recommended. |
1 working day |
| SS1021 |
DNA Sequencing Sample Quantification - Spectrophotometer Reading |
We provide this service of spectrophotometric analysis to ascertain sample purity and concentration before cycle sequencing. Combination of SS1020 and SS1021 is often recommended. |
1 working day |
| DNA Sequencing Custom Services |
| SS1022 |
DNA Sequencing Service - Clone PCR fragment into Linearised Vector |
Customer to provide fresh unpurified PCR products (50ul x 3 tubes). Clones delivered in plasmid or/ and glycerol stock available upon request. |
1 week |
| SS1023 |
DNA Sequencing Service - Clone PCR fragment into Circular Vector |
Customer to provide fresh unpurified PCR products (50ul x 3 tubes) and indicate the restriction enzyme (RE) to be used. Clones delivered in plasmid or/ and glycerol stock available upon request. |
1 week |
| SS1024 |
DNA Sequencing Service - Publication Quality |
We run the reactions twice to generate 2 different .ab1 files from the same DNA and its sequencing primer(s). Discounted price applies to the duplicate reaction. |
1 working day |
| SS1025 |
DNA Sequencing Service - Rolling Circle Amplification |
Hexamers are used to generate ssDNA from the circular DNA. The ssDNA is then available for cycle sequencing reaction. |
1-2 working days |
| DNA Sequencing Design and Analysis Services |
| SS1026 |
DNA Sequencing Analysis - BLAST |
Blast-N (nucleotides) from NCBI |
1 working day |
| SS1027 |
DNA Sequencing Analysis - Data Alignment with 2 sequences |
1 alignment data is generated from 2 DNA sequencing results (usually forward and reverse sequencing). |
1 working day |
| SS1028 |
DNA Sequencing Analysis - Data Alignment with 3 sequences |
1 alignment data is generated from 3 DNA sequencing results (usually forward, reverse & internal sequencing). |
1 working day |
| SS1029 |
DNA Sequencing Primer Design and Synthesis (Single primer) |
The sequencing primer is designed from 1 sequencing result. It usually apply for 1 directional sequencing, for the fragment size > 1.5 kbp. |
2-3 working days |
| SS1030 |
DNA Sequencing Primer Design and Synthesis (Forward and Reverse primer) |
The sequencing primers are designed from 2 sequencing results. It usually applies to bi-directional sequencing, for the fragment size > 3.0 kbp. |
2-3 working days |
| SS1042 |
DNA Sequencing Service - Dendrogram. Price per submission. |
1 dendrogram will be created using the sequencing traces generated by our Genetic Analyzers. |
1 working day |
| SS1043 |
DNA Sequencing Service - Dendrogram with previous data. Price per submission. |
Customer to provide other sequences to be included in this analysis. 1 dendrogram will be created using the provided sequences, which included the sequencing traces that not generated by our Genetic Analyzers. |
1 working day |
| Other Add-on Services |
| SS1031 |
DNA Sequencing Results in CD up to 700MB (Price per CD) |
Soft copy of results in CD. Soft copy is provided free of charge for customers who simply want to download the results in our link. |
1 working day |
| SS1032 |
Hardcopy Electropherogram Results (price per Reaction ID) |
Physical Colour printout of electropherogram. (Electropherograms are available for print through electronic files sent as part of your results package, at no additional cost.) |
1 working day |
| SS1033 |
Softcopy DNA Sequencing Report. Price per Order ID |
This sequencing report contains the data evaluation for each reaction according to the chemistry guide of BigDye® cycle sequencing by Applied Biosystems. |
1 working day |
| SS1034 |
Storage of Primers per 2 months for each tube. |
The sequencing primers will be discarded after 1 week from the completed order. A nominal charge applies if you need us to keep them in our laboratory. |
2 months |
| SS1036 |
DNA Sequencing Service - Drying of Ready to Load Samples. |
Price per Sample Residual ethanol on the precipitated BigDye® cycle sequencing will generate relatively low signal data. This service is available for the customers who are not able to dry the BigDye® reactions completely. |
1 working day |
| SS1037 |
Both Softcopy and Hardcopy DNA Sequencing Report. Price per Order ID |
Softcopy of DNA sequencing report to be sent through result link. This will be printed together with the coloured hardcopy DNA sequencing report. |
1 working day |
| SS1038 |
Storage of Samples per 2 months for each tube. |
All provided DNA sample(s) will be discarded after 1 week from the completed order. A nominal charge applies if you need us to keep them in our laboratory. |
2 months |
| SS1039 |
Preparation of Daughter Plate. |
Aliquot and deliver the remaining DNA sequencing samples in daughter plate. It is usually requested if the customer asks for SS1002 or/and SS1018. |
1 working day |
| SS1040 |
DNA Sequencing Service - OD Normalization prior DNA sequencing. Price per plate. |
OD normalization from 96-well plate before cycle sequencing. It is usually requested if the customer asks for SS1002 and/or SS1018. |
1 working day |
| SS1041 |
DNA Sequencing Service - Transformation of Plasmid DNA into E. coli. |
Services included 2 DNA sequencing reactions; and delivered in (i) 2 x 50uL of purified plasmid DNA and (ii) 2 x 1mL glycerol stock of cells. |
1 to 2 weeks |
| Expedite Service - Pickup |
Pick-up Charge for Expedite Service. One-time charge per order. |
Our Expedite Service is only available in selected locations. Please enquire if your location is covered. (Hyperlink to sequencing@base-asia.com) |
Request through 1oo before 9am, sample ready for pick-up before 11am. T&C apply. |
| Seq Expedite Service-SP |
DNA Sequencing Expedite Service, Single Pass Reaction. Price per reaction. |
Our Expedited Service is only available in selected locations. It is limited to 95 reactions per order and only available through our online ordering system (1oo) Not applicable if samples require add-on services e.g. clean-up, gel extraction before sequencing. |
Same day, before 6pm.
T&C apply. |
| Seq Expedite Service-RTL |
DNA Sequencing Expedite Service, Ready-to-load. Price per load. |
Our Expedited Service is only available in selected locations. It is limited to 95 loadings per order and only available through our online ordering system (1oo). Not applicable if samples require add-on services e.g. clean-up before sequencing. |
Same day, before 6pm.
T&C apply |
