Ready Mix DNA sequencing (SS1004)
A DNA Sequencing Service of pre-mixed samples and primers in a pre-determined quantity. With our Ready Mix Reaction Service, you can enjoy quality results at affordable prices. More importantly, you get total control over how your samples are submitted by mixing your own primer and DNA templates.
Due to the limited sample per reaction, no repeat is available. This service is not applicable for difficult templates and to certain regions. Please enquire on availability of the Ready Mix Reaction Sequencing Service from your local Sales Office.
Sample Preparation Requirements for Local Customers (Singapore and Malaysia) are as follows:
| Type of DNA |
Required DNA |
Primer |
Final volume |
| Purified PCR Product [90-250bp] |
10 ng in 5 µL |
10 pmoles in 1 µL |
6 µL |
| Purified PCR Product [251-500bp] |
20 ng in 5 µL |
10 pmoles in 1 µL |
6 µL |
| Purified PCR Product [>500bp] |
40 ng in 5 µL |
10 pmoles in 1 µL |
6 µL |
| Purified Plasmid |
200 ng in 5 µL |
10 pmoles in 1 µL |
6 µL |
| Purified M13/phagemid ssDNA |
100 ng in 5 µL |
10 pmoles in 1 µL |
6 µL |
| Purified Genomic DNA |
1000 ng in 3 µL |
30 pmoles in 1 µL |
4 µL |
Packing of Ready Mix DNA Sequencing Reaction Samples
- Mix sample and primer into 1.5ml microcentrifuge tube, 1 reaction per tube.
- Close lid tight and seal microcentrifuge tube with parafilm.
- Label side and lid of the tube with a permanent marker.
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