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Sample Preparation Requirements for DNA Sequencing Reactions for local customers (Singapore and Malaysia)
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| Type of DNA | Required Concentration & Volume | Primer Concentration & Volume / Rxn |
| Purified PCR Product [90-250bp] |
10 ng/µL min.10 µL per reaction in dH2O |
10 ng/µM or 10 pmol/µL, min 5 µL in dH2O per reaction |
| Purified PCR Product [251-500bp] | 20 ng/µL min.10 µL per reaction in dH2O | |
| Purified PCR Product [>500bp] | 40 ng/µL min.10 µL per reaction in dH2O | |
| Purified Plasmid | 100 ng/µL min.10 µL per reaction in dH2O | |
| Purified M13/phagemid ssDNA | 50 ng/µL min.10 µL per reaction in dH2O | |
| Purified Genomic DNA | 400 ng/µL min.20 µL per reaction in dH2O | 30 ng/µM or 30 pmol/µL, min 5 µL in dH2O per reaction |
For sample preparation requirements for Ready-Mix DNA Sequencing, please refer here
Sample Preparation Requirements for International Customers
We recommend that international customers send a larger volume of their samples to overcome loss of samples due to evaporation during the extended transportation to our facilities.
| Type of DNA | Required Concentration & Volume | Primer Concentration & Volume / Rxn |
| Purified PCR Product [90-250bp] |
10 ng/µL min.20 µL per reaction in dH2O |
10 ng/µM or 10 pmol/µL, min 10 µL in dH2O per reaction |
| Purified PCR Product [251-500bp] | 20 ng/µL min.20 µL per reaction in dH2O | |
| Purified PCR Product [>500bp] | 40 ng/µL min.20 µL per reaction in dH2O | |
| Purified Plasmid | 100 ng/µL min.20 µL per reaction in dH2O | |
| Purified M13/phagemid ssDNA | 50 ng/µL min.20 µL per reaction in dH2O | |
| Purified Genomic DNA | 400 ng/µL min.40 µL per reaction in dH2O | 30 pmol/µL or 30 /µM, min 10 µL in dH2O per reaction |
Packing of Samples (DNA templates and primers)
- Please prepare Sample in microcentrifuge tube (preferably in 0.5mL tubes) or directly on 96-well plate for high-throughput sequencing orders (empty the well no. H12 for our internal control reaction)
- Label clearly on the side and top of tube with a permanent marker
- Please use simple labels on your tubes (e.g. initials and numbers) and avoid long sample names
- Ensure that the lid is tightly closed and seal the tubes with parafilm
- Place samples in a plastic bag and attach the completed DNA Sequencing order form
Guidelines for packaging for safe transportation of DNA sequencing samples from Outstationed / International Customers:
- Please prepare Samples in microcentrifuge tube (preferably in 0.5mL tubes) or directly on 96-well plate sealed with strip-caps for high-throughput sequencing orders (empty the well no. H12 for our internal control reaction)
- Label clearly on the side and top of tube with a permanent marker
- Please use simple labels on your tubes (e.g. initials and numbers) and avoid long sample names
- Ensure that the lid is tightly closed and seal the lid with parafilm
- Place samples in a plastic bag. Do not stick samples directly onto order forms, please attach order form to the plastic bag instead.
- Wrap the plastic bag with a bubble bag and send in a padded envelope by overnight delivery either through Courier. DNA samples can be sent at room temperature.
- Notify 1st BASE via email and provide tracking number if any.
When in doubt, do not hesitate to contact us for any clarifications.
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Copyright © 2010. BASE Life Sciences Holdings. All rights reserved. |