DNA Sequencing Sample Preparation and Quantification
Maximise the success of your DNA Sequencing reaction by having 1st BASE perform the critical Sample Preparation and Quantification steps. The services we offer are listed below.
| Product No |
Name of Service |
| SS1012 |
DNA Sequencing Sample Preparation - PCR Clean-up |
| SS1013 |
DNA Sequencing Sample Preparation - PCR Clean-up 96-well format |
| SS1014 |
DNA Sequencing Sample Preparation - Gel Extraction by General Agarose |
| SS1015 |
DNA Sequencing Sample Preparation - Gel Extraction by General Agarose 96-well format |
| SS1016 |
DNA Sequencing Sample Preparation - Gel Extraction by PAGE |
| SS1017 |
DNA Sequencing Sample Preparation - Plasmid Extraction |
| SS1018 |
DNA Sequencing Sample Preparation - Plasmid Extraction 96-well format |
| SS1019 |
Ready to load Sequencing with Clean-up |
| SS1020 |
DNA Sequencing Sample Quantification - By Agarose Gel |
| SS1021 |
DNA Sequencing Sample Quantification - Spectrophotometer Reading |
Specifications
Sample Preparation Requirements for Local Customers (Singapore and Malaysia)
| Sample Preparation and Quantification Service |
Required Concentration & Volume |
| Quantification by UV Spectrometry (SS1021) |
: min. 15 µL for each sample |
| Quantification by Agarose Gel (SS1020) |
: min. 15 µL for each sample |
| Purification Using PCR Clean-up kit (SS1012/3) |
: 100 ng/µL, min 30 µL per reaction in dH2O |
| Purification Using Gel Extraction kit (SS1014/5) |
: 100 ng/µL, min 30 µL per reaction in dH2O |
| Ready-to-Load Sequencing with Clean-up (SS1019) |
: min. 10 µL, please indicate cycle reaction volume. |
Sample Preparation Requirements for International Customers
We recommend that international customers send a larger volume of their samples to overcome loss of samples due to evaporation during the extended transportation to our facilities.
| Sample Preparation and Quantification Service |
Required Concentration & Volume |
| Quantification by UV Spectrometry (SS1021) |
: min. 25 µL for each sample |
| Quantification by Agarose Gel (SS1020) |
: min. 25 µL for each sample |
| Purification Using PCR Clean-up kit (SS1012/3) |
: 100 ng/µL, min 40 µL per reaction in dH2O |
| Purification Using Gel Extraction kit (SS1014/5) |
: 100 ng/µL, min 40 µL per reaction in dH2O |
| Ready-to-Load Sequencing with Clean-up (SS1019) |
: min. 20 µL, please indicate cycle reaction volume. |
DNA Sequencing Sample Preparation - PCR Clean-up (SS1012, SS1013)
We accept PCR samples straight off the thermal cycler for template DNA purification. Samples will undergo a purification step using a silica PCR clean-up column kit. We accept either individual PCR reactions in single tubes or 96-well plate format.
DNA Sequencing Sample Preparation - Gel Extraction by General Agarose (SS1014, SS1015)
This is similar to the PCR Clean-up described above. Instead of purifying the PCR mix straight off the PCR reaction, they are first run on a gel. Then the target band is excised and DNA extracted using a silica gel extraction column kit.
DNA Sequencing Sample Preparation - Gel Extraction by PAGE (SS1016)
Similar to DNA Sequencing Sample Preparation – Gel Extraction (SS1014, SS1015) except that the medium for analysis is with polyacrylamide gels instead of agarose gels.
DNA Sequencing Sample Preparation - Plasmid Extraction (SS1017, SS1018)
We accept transformed bacterial cultures, grow them in our laboratory, and then extract the transformed plasmids using a plasmid prep kit prior to DNA sequencing. At the moment, we only accept stab culture for individual clones. 96-well format bacterial cultures are accepted as liquid cultures.
Ready-to-Load Sequencing with Clean-up (SS1019)
Customers may send us their pre-cycled sequencing reactions that are in single tubes or plate format. 1st BASE performs the post-cycle sequencing clean-up reaction for excess dye terminator removal and the subsequent analysis on our capillary electrophoretic analyzer.
DNA Sequencing Sample Quantification - By Agarose Gel (SS1020)
This additional quantification service provides customers with a means of verifying and quantifying their DNA sample prior to DNA sequencing. A small volume of DNA sample is run on an agarose gel to verify its purity and mass before proceeding with DNA Sequencing reactions.
DNA Sequencing Sample Quantification - Spectrophotometer Reading (SS1021)
This additional quantification service provides customers with a means of quantifying their DNA prior to DNA sequencing. A small volume of DNA sample is used to measure the OD260using the UV Spectrophotometer
|
