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DNA Sequencing Services
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   •   Single Pass DNA Sequencing (SS1001, SS1002)
   •   Ready-to-Load Sequencing (SS1005)
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   •   Primer Walking (SS1008, SS1009)
   •   Ready-to-Load DNA Sequencing with Clean Up (SS1019)
   •   DNA Sequencing Sample Preparation and Quantification
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Home > Products & Services > DNA Sequencing Services > Services Offered > DNA Sequencing Sample Preparation and Quantification >
DNA Sequencing Sample Preparation and Quantification

Maximise the success of your DNA Sequencing reaction by having 1st BASE perform the critical Sample Preparation and Quantification steps. The services we offer are listed below. 

 

Product No Name of Service
SS1012 DNA Sequencing Sample Preparation - PCR Clean-up
SS1013 DNA Sequencing Sample Preparation - PCR Clean-up 96-well format
SS1014 DNA Sequencing Sample Preparation - Gel Extraction by General Agarose
SS1015 DNA Sequencing Sample Preparation - Gel Extraction by General Agarose 96-well format
SS1016 DNA Sequencing Sample Preparation - Gel Extraction by PAGE
SS1017 DNA Sequencing Sample Preparation - Plasmid Extraction
SS1018 DNA Sequencing Sample Preparation - Plasmid Extraction 96-well format
SS1019 Ready to load Sequencing with Clean-up
SS1020 DNA Sequencing Sample Quantification - By Agarose Gel
SS1021 DNA Sequencing Sample Quantification - Spectrophotometer Reading

  

Specifications

 

Sample Preparation Requirements for Local Customers (Singapore and Malaysia)

 

Sample Preparation and Quantification Service Required Concentration & Volume
Quantification by UV Spectrometry (SS1021) : min. 15 µL for each sample
Quantification by Agarose Gel (SS1020) : min. 15 µL for each sample
Purification Using PCR Clean-up kit (SS1012/3) : 100 ng/µL, min 30 µL per reaction in dH2O
Purification Using Gel Extraction kit (SS1014/5) : 100 ng/µL, min 30 µL per reaction in dH2O
Ready-to-Load Sequencing with Clean-up (SS1019) : min. 10 µL, please indicate cycle reaction volume.

  

Sample Preparation Requirements for International Customers

We recommend that international customers send a larger volume of their samples to overcome loss of samples due to evaporation during the extended transportation to our facilities.  

Sample Preparation and Quantification Service Required Concentration & Volume
Quantification by UV Spectrometry (SS1021) : min. 25 µL for each sample
Quantification by Agarose Gel (SS1020) : min. 25 µL for each sample
Purification Using PCR Clean-up kit (SS1012/3) : 100 ng/µL, min 40 µL per reaction in dH2O
Purification Using Gel Extraction kit (SS1014/5) : 100 ng/µL, min 40 µL per reaction in dH2O
Ready-to-Load Sequencing with Clean-up (SS1019) : min. 20 µL, please indicate cycle reaction volume.


 

DNA Sequencing Sample Preparation - PCR Clean-up (SS1012, SS1013)

We accept PCR samples straight off the thermal cycler for template DNA purification. Samples will undergo a purification step using a silica PCR clean-up column kit. We accept either individual PCR reactions in single tubes or 96-well plate format. 

 

DNA Sequencing Sample Preparation - Gel Extraction by General Agarose (SS1014, SS1015) 

 This is similar to the PCR Clean-up described above. Instead of purifying the PCR mix straight off the PCR reaction, they are first run on a gel. Then the target band is excised and DNA extracted using a silica gel extraction column kit.

 

DNA Sequencing Sample Preparation - Gel Extraction by PAGE (SS1016)

Similar to DNA Sequencing Sample Preparation – Gel Extraction (SS1014, SS1015) except that the medium for analysis is with polyacrylamide gels instead of agarose gels.

 

DNA Sequencing Sample Preparation - Plasmid Extraction (SS1017, SS1018)

We accept transformed bacterial cultures, grow them in our laboratory, and then extract the transformed plasmids using a plasmid prep kit prior to DNA sequencing. At the moment, we only accept stab culture for individual clones. 96-well format bacterial cultures are accepted as liquid cultures.

 

Ready-to-Load Sequencing with Clean-up (SS1019)

Customers may send us their pre-cycled sequencing reactions that are in single tubes or plate format. 1st BASE performs the post-cycle sequencing clean-up reaction for excess dye terminator removal and the subsequent analysis on our capillary electrophoretic analyzer. 

 

DNA Sequencing Sample Quantification - By Agarose Gel (SS1020)

This additional quantification service provides customers with a means of verifying and quantifying their DNA sample prior to DNA sequencing. A small volume of DNA sample is run on an agarose gel to verify its purity and mass before proceeding with DNA Sequencing reactions. 

 

DNA Sequencing Sample Quantification - Spectrophotometer Reading (SS1021)

This additional quantification service provides customers with a means of quantifying their DNA prior to DNA sequencing. A small volume of DNA sample is used to measure the OD260using the UV Spectrophotometer

 

 
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