1st BASE DNA Sequencing Service
By utilizing Applied Biosystems's highest capacity-based genetic analyzer platforms and optimized protocols for various template requirements, we give superior data without compromising on turnaround time. For DNA sequencing reactions, we adopt BigDye? Terminator v3.1 cycle sequencing kit chemistry, the latest chemistry that is based on Sanger's dideoxy sequencing.
We pride ourselves for giving you industry-leading services. Our Quality Control standards are based on QV, trace score and contiguous read length. The reads we generate are benchmarked against leading industry providers. Our vast experience also allows us to achieve highest success rates even with difficult template. Our strong technical support ensures that you get quality results and prompt assistance in terms of troubleshooting.
Features of 1st BASE DNA Sequencing Services:- High quality, long read lengths with fast turnaround time
- Wide range of free sequencing primers for ABI's dye terminator chemistry
- 1st BASE DNA Sequencing Collection Kit available for ease of organizing DNA templates/primers
- Email notification of sequencing results, electropherogram and sequences
- FREE automatic re-load of failed reaction(s)
Types of DNA Sequencing Services
| Product No. | Name of Service | Turnaround Time |
|---|---|---|
| DNA Sequencing Services | ||
| SS1001 | Single Pass DNA Sequencing | 1-2 working days |
| SS1002 | Single Pass DNA Sequencing, 96-well format | 2-3 working days |
| SS1003 |
|
1-2 working days |
| SS1004 | Ready Mix DNA Sequencing | 1-2 working days |
| SS1005 | Ready to Load Sequencing | 1-2 working days |
| SS1006 |
Difficult
template Sequencing (70% GC-rich/ 70% AT-rich/ GT-rich/ repetitive sequence) |
2-3 working days |
| SS1007 | Bi-Directional DNA Sequencing | 1-2 working days |
| SS1008 | Primer Walking of Constructs Service - Single Pass | 1.5kb per week |
| SS1009 | Primer Walking of Constructs Service - Bi-Directional | 1.5kb per week |
| SS1010 | Direct Genomic Sequencing | 2-3 working days |
| SS1011 | BAC (Bacteria Artificial Chromosome) End Sequencing | 2-3 working days |
| Sample Preparation and Quantification Service | ||
| SS1012 | DNA Sequencing Sample Preparation - PCR Clean-up | 1-2 working days |
| SS1013 | DNA Sequencing Sample Preparation - PCR Clean-up 96-well format | 2-3 working days |
| SS1014 | DNA Sequencing Sample Preparation - Gel Extraction by General Agarose | 1-2 working days |
| SS1015 | DNA Sequencing Sample Preparation - Gel Extraction by General Agarose 96-well format | 2-3 working days |
| SS1016 | DNA Sequencing Sample Preparation - Gel Extraction by PAGE | 1-2 working days |
| SS1017 | DNA Sequencing Sample Preparation - Plasmid Extraction | 2-3 working days |
| SS1018 | DNA Sequencing Sample Preparation - Plasmid Extraction 96-well format | 2-3 working days |
| SS1019 | Ready to load Sequencing with Clean-up | 1 working day |
| SS1020 | DNA Sequencing Sample Quantification - By Agarose Gel | 1 working day |
| SS1021 | DNA Sequencing Sample Quantification - Spectrophotometer Reading | 1 working day |
| DNA Sequencing Custom Services | ||
| SS1022 | DNA Sequencing Service - Clone PCR fragment into Linearize Vector | 1 week |
| SS1023 | DNA Sequencing Service - Clone PCR fragment into Circular Vector | 1 week |
| SS1024 | DNA Sequencing Service - Publication Quality | 1 working day |
| SS1025 | DNA Sequencing Service - Rolling Circle Amplification | 1-2 working days |
| DNA Sequencing Design and Analysis Services | ||
| SS1026 | DNA Sequencing Analysis - BLAST | 1 working day |
| SS1027 | DNA Sequencing Analysis - Data Alignment with 2 sequences | 1 working day |
| SS1028 | DNA Sequencing Analysis - Data Alignment with 3 sequences | 1 working day |
| SS1029 | DNA Sequencing Primer Design and Synthesis (Single primer) | 2-3 working days |
| SS1030 | DNA Sequencing Primer Design and Synthesis (Forward and Reverse primer) | 2-3 working days |
| SS3001 | NEW Fragment Analysis with Run Report | 3-4 working days |
| SS3002 | NEW Fragment Analysis with Run Report, 96-well format | 4-5 working days |
| SS3003 | NEW Fragment Analysis | 3-4 working days |
| SS3004 | NEW Fragment Analysis, 96-well format | 4-5 working days |
| SS3005 | NEW Fragment Analysis Re-Load - Single Well / Tube | 2 working days |
| SS3006 | NEW Fragment Analysis Spectral Optimization - Single Dye | 3 working days |
| SS3007 | NEW Fragment Analysis Spectral Optimization - Two Dyes | 3 working days |
| SS3008 | NEW Fragment Analysis Spectral Optimization - Dye Set D | 3 working days |
| SS3009 | NEW Fragment Analysis Spectral Optimization - Dye Set G5 | 3 working days |
| SS3010 | NEW Fragment Analysis Sample Quantification - By Agarose Gel | 1-2 working days |
| SS3011 | NEW Fragment Analysis Sample Quantification - By Spectrophotometer Reading | 1-2 working days |
| Other Add-on Services | ||
| SS1031 | DNA Sequencing Results in CD up to 700MB (Price per CD) | 1 working day |
| SS1032 | Hardcopy Electropherogram Results Price per Reaction ID | 1 working day |
| SS1033 | Softcopy DNA Sequencing Report. Price per Order ID | 1 working day |
| SS1034 | Storage of Primers per 2 months for each tube. | |
| SS1035 | Urgent Process of Order | 24 hours |
| SS1036 | DNA Sequencing Service - Drying of Ready to Load Samples. Price per Sample | 1 working day |
| SS1037 | Both Softcopy and Hardcopy DNA Sequencing Report. Price per Order ID. | 1 working day |
| SS1038 | Storage of Samples per 2 months for each tube. | |