1st BASE CRISPR Screening Services

Want to speed up your CRISPR experiments?HRMT7E1 AssaySanger SequencingNGSFLA-PCR
 

T7E1 Mismatch Cleavage Assay

Mismatch Assay Mutation Screening Service includes:

  • QC of gDNA from targeted and control cells
  • Design and synthesis of PCR primers to amplify wild-type cell gDNA and edited type cell gDNA
  • PCR optimization, PCR set up and Clean up
  • T7E1 digestion for Heteroduplexes formation
  • Heteroduplexes digestion and QC
  • Deliverables: Gel electrophoresis Analysis Report

 

HRM (High-Resolution Melting)

HRM qPCR Mutation Screening Service includes:

  • QC of gDNA
  • Design and synthesis of PCR primers to amplify region containing target site
  • PCR with reagent containing HRM fluorescent dye
  • Melt curve generation
  • Deliverables: HRM amplification curve, Normalized melt graph, Different graph, and HRM melt analysis & HRM analysis report

 

Sanger Sequencing

Sanger Sequencing Screening Service includes:

  • QC of Plasmid/PCR amplicon
  • BigDye® Terminator v3.1 Cycle Sequencing Reaction set up and Post Sequencing clean up
  • ABI 3730XL Sequencer run
  • Deliverables: Electropherogram (.ab1 format) and sequences (.seq fasta format)

 

NGS(Next Generation Sequencing)

Next Generation Sequencing Mutation Screening Service includes:

  • QC of gDNA
  • Design and synthesis of PCR primers to amplify region containing target site
  • Library preparation and QC
  • MiSeq 150/250/300 PE reads run
  • Deliverables: Raw Data and after run QC Analysis Report

 

FLA-PCR(Fragment Length Analysis)

FLA-PCR Mutation Screening Service includes:

  • QC of gDNA
  • Design and synthesis of PCR primers to amplify wild-type cell gDNA and edited type cell gDNA
  • PCR optimization, PCR set up and Clean up
  • FA RUN set up using LIZ dye marker
  • Deliverables: Efficiency of edits Analysis Report

 

Review Article

Visit this link for more information: http://base-asia.com/snp-genotyping-mutation-screening

 

How to Order

Please enquire for and complete the 'CRISPR Screening Order Form'. Send your completed form to the respective email addresses: 

Location Singapore Malaysia International
Email to: mbs@axilscientific.com mbs@apicalscientific.com mbs@apicalscientific.com
Tel: +65 67757318 +60-3-8943 3252 +60-3-8943 3252