Species Barcoding
Utilising our expertise in DNA sequencing, 1st BASE now offers barcoding/identification services for pure isolates of bacterial, fungal, plant and animal samples. Molecular methods will be employed to give more accurate and reproducible results and even identifying the samples down to the genus level. For barcoding/identification services of mixed samples, please enquire through our Custom Research Services (CRS).
Appropriate controls are used at all levels to ensure the results are consistent and of the highest quality. All reports include the assembled sequences of your samples and the alignment of the top 10 hits to GenBank databases.
For other species, please enquire.
Features
- Barcoding and identification services for isolated pure samples
- Accurate and reproducible results
- Fast turnaround time
- Bidirectional sequencing prevents ambiguity in sequence
- Sequences are matched against a standard database, or a database of choice
- Comprehensive reports include sequences of sample, and the top 10 Hits of the BLAST results
- Additional analyses e.g. Phylogenetic analysis available
- NEW Free Neighbor Joining (unrooted tree)
Protocol
- PCR Amplification of conserved regions of gDNA sample
- Purification of amplicons
- Bi-directional sequencing of amplicons
- Data alignment and BLAST of results
Specifications
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Product No.
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Description
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MBS-5001
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Bacterial Species Barcoding, 16 sRNA, partial sequence (~ 600bp)
Includes:
- PCR amplification and sequencing
- Data analysis (BLAST to show the top 10 matches from database)
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MBS-5002
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Bacterial Species Barcoding, 16 sRNA, full-length sequence (~ 1500bp)
Includes:
- PCR amplification and purification
- Bidirectional PCR product sequencing
- Data analysis (BLAST to show the top 10 matches of each isolate from database)
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|
MBS-5003
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Plant Species Barcoding, rbcl (~ 600bp) and matK (~850 bp)
Includes:
- PCR amplification and purification
- Bidirectional PCR product sequencing
- Data analysis (BLAST to show the top 10 matches from database)
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|
MBS-5004
|
Fungal Species Barcoding ITS (~ 700bp)
Includes:
- PCR amplification and purification
- Bidirectional PCR product sequencing
- Data analysis (BLAST to show the top 10 matches from database)
|
| MBS-5005 |
Animal Species Barcoding, COI (~ 700bp)
Includes::
- PCR amplification and purification
- Bidirectional PCR product sequencing
- Data analysis (BLAST to show the top 10 matches from database
|
| MBS-500x - Proviso |
Species Barcoding - Pure gDNA isolate from bacteria, fungus, plant or animal
A minimum set-up fee is chargeable when there is no PCR result.
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| MBS-5200 |
Species Barcoding
gDNA mini prep for plant, bacteria, fungus (Solutions A and B will be provided)
|
| MBS-5201 |
Species Barcoding
gDNA mini prep for animal (Solutions A, TP and B will be provided)
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