High Performance Reagents for Routine PCR
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Product Information
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Product No.
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Product Description
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Product Details
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BIO-5110-500U
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Taq DNA Polymerase (recombinant), MgCl2 separate, 500U
Supplied with 10x Taq Buffer and 25mM MgCl2
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| BIO-5110-2500U |
Taq DNA Polymerase (recombinant), MgCl2 separate, 2500U
Supplied with 10x Taq Buffer and 25mM MgCl2
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BIO-5120-1ml
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dNTP Mix, 10mM each, 1.0ml
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BIO-5125-1ml
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dNTP Set, 100mM, 4 X 0.25ml
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C/No. BIO-5110-500U Taq DNA Polymerase (recombinant) - MgCl2 separate, 500U
Description
Taq DNA Polymerase is a thermostable DNA polymerase that catalyzes 5'→3' synthesis of DNA, has zero detectable 3'→5' exonuclease (proofreading) activity and possesses minimal 5'→3' exonuclease activity. In addition, Taq DNA Polymerase exhibits deoxynucleotidyl transferase activity, which frequently results in the addition of extra adenines at the 3'-end of PCR products. Recombinant Taq DNA Polymerase is ideal for standard PCR of templates 5kb or shorter.
Source
An E.coli strain that carries a cloned pol gene from Thermus aquaticus.
Unit Definition
One unit of the enzyme catalyzes the incorporation of 10nmol of deoxyribonucleotides into a polynucleotide fraction in 30min at 74°C.
Applications
- DNA sequencing
- DNA labeling
- PCR for cloning
- PCR amplification of DNA fragments up to 5kb
Supplied with 10x Taq Buffer and 25mM MgCl 2
Buffer Composition
Storage buffer
| 20mM Tris-HCl |
0.1mM EDTA |
| 0.5% (v/v) Nonidet P40 |
1mM DTT |
| 0.5% (v/v) Tween 20 |
100mM KCl |
| 50% (v/v) Glycerol |
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10X Taq buffer without MgCl 2
750mM Tris-HCl (pH 8.8 at 25°C)
200mM KCl
50mM (NH 4) 2SO 4
0.5% (v/v) Nonidet P40
Remarks
- Half-life of this enzyme is >40mins @ 95°C.
- Taq DNA Polymerase accepts modified nucleotides (e.g. biotin-, digoxigenin-, fluorescent-labeled nucleotides) as substrates for the DNA synthesis .
C/No. BIO-5120-1ml dNTP Mix, 10mM each
Description
Mixture contains aqueous solutions of the following at a final concentration of 10mM.
dATP
dTTP
dCTP
dGTP
With reduced number of pipetting steps, dNTPs remains highly pure which ensure successful PCR as the presence of contaminants can result in a decrease in amplification sensitivity and product yield.
Applications
- DNA sequencing
- DNA labeling
- PCR
- Long PCR
- RT-PCR
- cDNA synthesis
- Primer extension
C/No. BIO-5125-1ml dNTP Set, 100mM each
Description
Contains 4 separate tubes of the following aqueous solutions at a concentration of 100mM each in 0.25ml.
2’-Deoxyadenosine-5’-triphosphate trisodium salt (dATP)
2’-Deoxythymidine-5’-triphosphate trisodium salt (dTTP)
2’-Deoxycytidine-5’-triphosphate trisodium salt (dCTP)
2’-Deoxyguanosine-5’-triphosphate trisodium salt (dGTP)
Applications
- DNA sequencing
- DNA labeling
- PCR
- Long PCR
- RT-PCR
- cDNA synthesis
- Primer extension
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