Reagents for DNA Electrophoresis
ExactMark 100bp DNA Ladder, Ready-To-Use (BIO-5130)
Description Used for sizing and approximate quantification of wide range double-stranded DNA on agarose gel. The ladder is composed of eleven individual DNA fragments (in base pairs): 1500, 1000, 900, 800, 700, 600, 500, 400, 300, 200 and 100. It contains two reference bands of 1500 and 500bp for easy orientation. Orange G and xylene cyanol FF are used as tracking dyes. Storage Buffer 10mM Tris-HCl (pH 8.0) Protocol for Loading - Load 5µl of the solution into a 5mm agarose gel lane.
Recommendations - Do not heat solution prior to loading. |
ExactMark 1kb DNA Ladder, Ready-To-Use (BIO-5140)
Used for sizing and approximate quantification of wide range double-stranded DNA on agarose gel. The ladder is composed of thirteen individual DNA fragments (in base pairs): 10000, 8000, 6000, 5000, 4000, 3000, 2500, 2000, 1500, 1000, 750, 500, and 250. It contains three reference bands of, 3000 and 1000bp for easy orientation. Bromophenol blue is used as tracking dye. Storage Buffer 10mM Tris-HCl (pH 8.0) Protocol for Loading - Load 5µl of the solution into a 5mm agarose gel lane.
Recommendations - Do not heat solution prior to loading. |
Tri-Color 6X DNA Loading Dye (BIO-5160-6ml)
Used for loading DNA markers and samples on agarose or polyacrylamide gels. It contains three dyes – Orange G, Xylene cyanol FF and Bromophenol blue. This allows better visual tracking of DNA migration during electrophoresis. 6X DNA Loading Dye
Protocol for Loading - Mix 1 part of dye solution with 5 parts of DNA sample. |
FloroSafe DNA Stain, 1ml (BIO-5170-1ml)
Non-carcinogenic alternative of Ethidium Bromide, DNA Stain can be used as a safer alternative compared to the traditional ethidium bromide stain used for DNA and RNA detection in agarose gel. Compared to ethidium bromide, it has low toxicity and mutagenicity. DNA Stain has two secondary fluorescence excitation peaks (~270 nm; ~295 nm) and one strong excitation peak centered around 490 nm. The fluorescence emission is similar to Ethidium Bromide when bound to DNA which is at about 530 nm. Usage Protocols Precasting method: - Prepare 100ml of 0.8-3.0% agarose gel solution. Post-staining method: - For agarose gel less than 0.5cm thickness, 10 – 25µl of the stain should be used per 100ml of buffer |
Product Information
Product No. |
Description |
No of Applications |
Detail |
MSDS |
BIO-5130-50ug |
ExactMark 100bp DNA Ladder, Ready-To-Use, 50ug |
~100 applications |
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BIO-5130-100ug |
ExactMark 100bp DNA Ladder, Ready-To-Use, 100ug |
~200 applications |
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BIO-5140-50ug |
ExactMark 1kb DNA Ladder, Ready-To-Use, 50ug |
~100 applications |
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BIO-5140-100ug |
ExactMark 1kb DNA Ladder, Ready-To-Use, 100ug |
~200 applications |
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BIO-5140-250ug |
ExactMark 1kb DNA Ladder, Ready-To-Use, 250ug |
~500 applications | Protocol | |
BIO-5160-6ml |
Tri-Color 6X DNA Loading Dye, 6 X 1ml |
6000 applications |
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BIO-5170-1ml |
FloroSafe DNA Stain, 1ml |
~200 applications (based on precasting method) |
Download Now | |
BIO-5171-600ul | Floro+ Red Nucleic Acid Stain, 600ul | ~200 applications (based on precasting method) | Protocol | Download Now |
BIO-5172-600ul | Floro+ Green Nucleic Acid Stain, 600ul | ~200 applications (based on precasting method) | Protocol | Download Now |
See also BUF - Product Listing for our Listing of popular buffers for DNA Electrophoresis.