Specifications
Standard 2 rabbits 8-week Anti-sera Production Schedule (Accelerated Schedule)
| Week |
Procedure |
| 0 |
· Pre-immunization bleed:
To collect 5mL of pre-immune serum (negative control) per rabbit
· Primary immunization:
Each rabbit is injected with 200 µg of antigen emulsified in Complete Freund's Adjuvant (CFA)
|
| 2 |
· Immunization No. 2:
100 µg antigen per rabbit in Incomplete Freund's Adjuvant (IFA) |
| 4 |
· Immunization No. 3:
100 µg antigen per rabbit in Incomplete Freund's Adjuvant (IFA) |
| 5 |
· Production Bleed No. 1:
25mL serum per rabbit with ELISA Testing and Reporting |
| 6 |
· Immunization No. 4:
100 µg antigen per rabbit in Incomplete Freund's Adjuvant (IFA) |
| 7 |
· Production Bleed No. 2:
25mL serum per rabbit
|
| 8 |
· Production Bleed No. 3:
25mL serum per rabbit (terminal bleed) |
Upon the project completion based on the packages, we offer the following options to the customer:
- Termination: This will take effect upon confirmation at no extra cost.
- Project Extension:
- Rabbits:
28 Days
1 immunization
2 production bleeds (20-25 ml each)
- Chickens:
28 Days
1 immunization
2 production bleeds (1 ml each)
2 egg collection periods
- Goat:
28 Days
1 immunization
* 2 production bleeds (250 ml each)
- Exsanguination: Includes a series of two bleeds for a total of about 60mL per rabbit
- Maintenance: Rabbits are kept (no immunizations or bleeds) until further notice
Below is a summary of the packages available for your reseach needs. For full package details, please click here.
Polyclonal Antibody Package List
Service
Name |
Deliverables |
| Peptide Synthesis (15aa, > 70% purity) |
Pre-immune Serum |
Serum |
Affinity-Purified Antibody |
ELISA Report |
| PAb 8 week- RAB1 (2 Rabbits) |
Immunogen provided by researcher |
Each rabbit 5 ml (total 2 x 5ml) |
Yes |
Nil |
Each rabbit on Bleed 1 |
| PAb 8 week- RAB2 (2 Rabbits) |
5mg |
| PAb 8 week- RAB3 (2 Rabbits) |
Yes
|
Yes
|
| PAb 8 week- RAB4 (2 Rabbits) |
Nil |
Yes
|
Redi – Rabbit™ Antibody Package List
Service
Name |
Deliverables |
| Peptide Synthesis (15aa, > 70% purity) |
Pre-immune Serum |
Control Antibody (Protein A purified pre-immune serum from project-specific rabbits) |
Serum |
Affinity-Purified Antibody with Guaranteed Yields |
ELISA Report |
| Redi Option 1 |
5mg |
Each rabbit 5 ml (total 2 x 5ml) |
3mg |
Nil |
3mg |
Nil |
| Redi Option 2 |
5mg |
| Redi Option 3 |
5mg |
10mg |
| Redi Option 4 |
20mg |
Phospho – Specific Rabbit Antibody Package List
Service
Name |
Deliverables |
| Phospho peptide synthesis (15aa, > 90% purity) |
Non-phospho peptide synthesis (15aa, > 70% purity) |
Pre-immune Serum |
Serum |
Affinity-Purified Antibody |
ELISA Report |
| Rabbit Package 2P |
5mg |
Nil |
Each rabbit 5 ml (total 2 x 5ml) |
Yes
|
Nil |
Each peptide on Bleed 1 |
| Rabbit Package 2PN |
5mg |
| Rabbit Package 3PN |
Yes
|
Yes
|
| Rabbit Package 4PN |
Nil |
Yes
|
Peptide Cocktail Rabbit Antibody Package List
Service
Name |
Deliverables |
| Synthesis of peptides (15aa, > 70% purity) |
Pre-immune Serum |
Serum |
Affinity-Purified Antibody |
ELISA Report |
| Rabbit Package 2PC2 |
2 peptides (5mg each) |
Each rabbit 5 ml (total 2 x 5ml) |
Yes
|
Nil |
Each peptide on Bleed 1 |
| Rabbit Package 3PC2 |
Yes
|
Yes
|
| Rabbit Package 2PC3 |
3 peptides (5mg each) |
Yes
|
Nil |
| Rabbit Package 3PC3M |
Yes
|
Yes
|
|
