Custom Antibody Production
At 1st BASE, we are proud to offer a wide range of customised services for Antibody Production. A committed team of scientists is on hand to provide timely and personalised services for your projects. We have put together several Custom Antibody Packages for both Monoclonal and Polyclonal Antibodies to make it even easier to order with us.
| MAb Packages and Services |
| MAb Production Package 1A |
Custom MAb Production Package 1A, includes:
(Immunogen provided by researcher)
* Phase I: immunization of 5 mice (10-16 weeks)
* Phase II: fusion of mouse spleen with myeloma cells (2-3 weeks)
* Phase IIIa: ELISA screening and Subclone of 3 positive wells (4-6 weeks)
Final Clone Isotyping on each final clone provided, confirming Ig class
|
| MAb Production Package 1B |
Custom MAb Production Package 1B, includes:
(Immunogen provided by researcher)
* Phase I: immunization of 5 mice (10-16 weeks)
* Phase II: fusion of mouse spleen with myeloma cells (2-3 weeks)
* Phase IIIa: ELISA screening and Subclone of 3 positive wells (4-6 weeks)
* Phase IIIb: Screening of positive wells by Western Blotting (WB) or Immunocytochemistry (ICC)
Final Clone Isotyping on each final clone provided, confirming Ig class
|
MAb Production Package 2A |
Custom MAb Production Package 2A, includes:
Peptide synthesis (HPLC >90%, <25 aa) & Conjugation
* Phase I: immunization of 5 mice (10-16 weeks)
* Phase II: fusion of mouse spleen with myeloma cells (2-3 weeks)
* Phase IIIa: ELISA screening and Subclone of 3 positive wells (4-6 weeks)
Final Clone Isotyping on each final clone provided, confirming Ig class
|
| MAb Production Package 2B |
Custom MAb Production Package 2B, includes:
Peptide synthesis (HPLC >90%, <25 aa) & Conjugation
* Phase I: immunization of 5 mice (10-16 weeks)
* Phase II: fusion of mouse spleen with myeloma cells (2-3 weeks)
* Phase IIIa: ELISA screening and Subclone of 3 positive wells (4-6 weeks)
* Phase IIIb: Screening of positive wells by Western Blotting (WB) or Immunocytochemistry (ICC)
Final Clone Isotyping on each final clone provided, confirming Ig class
|
| PAb Packages and Services |
| PAb 8-week Package 1 |
Polyclonal Antibody Production - 2 Rabbits
(Immunogen provided by researcher)
Includes:
- Immunization and anti-sera production (75mL/rabbit)
- ELISA Testing for Bleed 1 |
| PAb 8-week Package 2 |
Polyclonal Antibody Production - 2 Rabbits
Includes:
- Peptide synthesis (15aa),Purification (> 70% purity) and Conjugation
- Immunization and anti-sera production (75mL/rabbit)
- ELISA Testing for Bleed 1
|
| PAb 8-week Package 3 |
Polyclonal Antibody Production - 2 Rabbits
Includes:
- Peptide synthesis (15aa), Purification (> 70% purity) and Conjugation
- Immunization and anti-sera production (62.5mL/rabbit)
- Affinity Purification on Bleed 3 for 12.5mL/ rabbit (Includes reusable column)
- ELISA Testing on Bleed 1
|
PAb 8-week Package 4 |
Polyclonal Antibody Production - 2 Rabbits
Includes:
- Peptide synthesis (15aa), Purification (> 70% purity) and Conjugation
- Immunization and anti-sera production
- Large scale Affinity Purification for all Bleeds (75mL/rabbit)
- ELISA Testing on Bleed 1 |
Other related analysis services are also available. They include:
- Peptide Synthesis and Purification
- Peptide Conjugation
- ELISA Testing and Reporting
- Affinity Purification (Standard 25ml or Large Scale 250ml)
- Affinity Column Preparation
- Protein A/G Purification
- Rabbit Maintenance
- Project Extension
- Rabbit Exsanguination
|
