1st BASE Custom Polyclonal Antibody Production Services
1st BASE provides a complete range of services for your polyclonal antibody project. We offer full custom antibody production from the design assistance of suitable peptide epitopes and peptide synthesis to affinity purification of the serum.
Stringent monitoring in quality and titer checking by ELISA will be conducted in each project to ensure specificity and sufficient amounts of your polyclonal antibody for your applications. 1st BASE now launches an Accelerated Rabbit Antibody Production - only 8 weeks to complete!
Features of Anti-sera Production Project:- Free Design Assistance and Antigenicity Analysis for Peptide Antibody projects.
- Two New Zealand White (female) rabbits will be immunized for each immunogen
- Accelerated eight-week immunization schedule New!
- Four boosts and a single ELISA testing are included in our standard programme
- Three production bleeds per rabbit for a final total yield of around 150ml
8-week Anti-sera Production Schedule (Accelerated Schedule)
| Week | Procedure |
|---|---|
| 0 | · Pre-immunization bleed: To collect 5mL of pre-immune serum (negative control) per rabbit · Primary immunization: Each rabbit is injected with 200 µg of antigen emulsified in Complete Freund's Adjuvant (CFA) |
| 2 | · Immunization No. 2: 100 ng antigen per rabbit in Incomplete Freund's Adjuvant (IFA) |
| 4 | · Immunization No. 3: 100 ng antigen per rabbit in Incomplete Freund's Adjuvant (IFA) |
| 5 | · Production Bleed No. 1: 25mL serum per rabbit |
| 6 | · Immunization No. 4: 100 ng antigen per rabbit in Incomplete Freund's Adjuvant (IFA) |
| 7 | · Production Bleed No. 2:
25mL serum per rabbit ELISA Testing and Reporting |
| 8 | · Production Bleed No. 3: 25mL serum per rabbit (terminal bleed) |